I want to conjugate Streptavidin to the surface of gold nanoparticles and gold nanorods. The surface chemistry of gold nanoparticles is citrate whereas the surface chemistry of gold nanorods is CTAB. Any suggestion? Thanks
You can use covalent attachment of streptavidin to Gold NPs using EDC chemistry. Hope this attached paper will help you.
if u want to conjugate the streptavidin. first thing u need to remember that, whether surface of Au has either if the group like -OH, -COOH, NH2, if you had modified with the above mentioned group then you can use EDC- NHS chemistry to covalently bind Streptavidin to the gold nano particle. remember pH definitely play an role in this reaction.
Here is a protocol : http://www.ncbi.nlm.nih.gov/pubmed/20012851
best,
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