How to use less antibody in a Western blot?

I'm trying to figure out how to not need so much antibody for our Western blots. Right now, we use 10 mL of buffer to dilute our antibodies in. Any less than that, and the membrane isn't submerged...

27 August 2019 8,607 3 View

Can you help me to create a fuzzy model for interaction IoT devices?

I have probleme to build a fuzzy model/controller for organisation interaction devices in IoT network!

18 June 2019 2,681 3 View

Primer Concentration of RT?

Hello, I am using a gene speciic primer to perform reverse transcription. I need to use 20pmols of my primer in a final reaction volume of 20ul. Is 20pmol interpreted as 20pmol/uL (20uM) in...

28 October 2018 7,946 4 View

What is the relationship between Intelligence and Learning?

Describe the relationship between intelligence and learning? May you please provide journals or articles about this matter. Thanks!

13 June 2018 2,441 8 View

Are there limitations when using AMOS or the PROCESS macro for SPSS to test for moderation?

I've often heard that there is virtually no reason why someone would prefer using AMOS to test for moderation than PROCESS and that it is overall more troublesome. Some even argue that PROCESS is...

01 November 2015 1,282 16 View

Wich protocol are you using to stain frozen liver sections with oil red o ?

I have tried till today a lot of protocols (IHC world,...), using isopropanol or propylene glycol or both, but I haven't had any satisfaying results yet...The stain is scattering everywhere out of...

07 September 2015 6,449 7 View

Why do I see "filaments" in my adhesion assays ?

04 March 2015 7,209 2 View

Can someone please help me understand some aspects of conducting a factor analysis please?

I have collected my data and I am now analyzing it. Here are the areas I cannot understand: 1. The pre-post tests questions are alike every way (except in how participants respond after exposure...

18 October 2014 9,540 7 View

Is there any difference in teaching in a University to teaching in a Community College?

I'm being asked if I've ever taught in a Community College as a precursor to a learning/design discussion. There seems to be a belief that teaching and learning in Community Colleges today, are...

30 April 2014 6,878 9 View

What is the best way to induce an s.aureus skin infection in a mouse model?

I'm researching on the effects of plant antimicrobials in S.aureus skin infections, what could be the best way to place the S.aureus?

29 January 2014 9,404 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View