How to best represent qPCR DD Ct or D Ct as a box plot?

I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...

11 February 2021 7,228 3 View

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26 December 2020 9,337 3 View

PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

How to solve MSTRAT software Running problem?

Hello, we have been trying to run MSTRAT Software for developing a core collection of Chilean Garlic genetic resources, but we keep getting the error: ether couldn't execute...

25 November 2020 9,748 2 View

DNA disappears from gel, whats going on?

Recently I've been doing some PCRs and the primes used to work a month ago, but now that we want to repeat the experiment, they are not working anymore. I have noticed something in my gels, and it...

28 October 2020 4,427 4 View

What books on anticancer potential of polysaccharides obtained from fungi recommend me to read?

I am investigating the medicinal potential of the polysaccharides present in edible fungi native to my country and that can help prevent or treat some types of cancer.

16 February 2020 7,775 3 View

Any behavior test for social novelty apart from 3 chamber test?

Any behavior test for social novelty apart from 3 chamber test? I want to look if there is an impairment in social novelty or social memory (in rats). I wanted to know if apart from the 3 chamber...

11 November 2019 9,876 3 View

How to cite chinese names?

This may look like an odd question, but since I'm latinamerican I'm not familiar with chinese names, the usual way to reference names is by writting the last name and the initial of the names, but...

11 September 2019 9,386 3 View

Does anyone knows this plant?

I need samples of a plant named Kermadecia sinuata, according to the data I got it is found in New Caledonia, anyone knows any repository of exotic species that could have this specimen and that...

12 February 2019 2,125 2 View

How to separate very closed PCR bands?

I've been trying to purifiy my electrophoresis bands but they are very closed to each other, I've tried 2% agarose gels, ran at 90V for 2 hours and this is as far as I can get them to separate. My...

07 November 2018 5,383 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View