How can I calculate the volume of sample to be loaded with desired concentration in wells after DC Assay?

06 June 2015 1 4K Report

Hi Everyone,

Can anyone help me in calculating the lysate volume of required concentration to be loaded in wells after DC Assay for western blot analysis?

Marcos Ucha Tortuero

After the DC assay you know the protein concentration of your sample and you can calculate the volume using this formula:

Volume of sample to load(ul)=Amount of protein desired(ug)/concentration of your sample(ug/ul)

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