http://www.sciencedirect.com/science/article/pii/S0021979714003397
It depends a bit on the concentration range that you wish to investigate. At low concentrations, you should be able to use the standard analytical chemistry approach. First obtain, either by experiment or from literature, a calibration line of absorption versus concentration. Then you can determine for an unknown sample the absorption and via the calibration line find the concentration.
However, for high concentrations, the absorption does not vary a lot anymore with concentration and so the method becomes much less accurate. If possible, one can dilute the sample until it comes in a suitable range of absorptions. A bit less accurate but still doable.
For the absorption values one takes a well-defined peak and integrates it over wavelength to get the overall absorption in that band.
You should find more information in a decent analytical chemistry book.
Thank you very much for your answers Mr. Ger Koper and Mrs. Bojidarka B.vanova.
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