I have been working on antioxidant activity using FRAP assay. However, the calculation for FRAP assay is
FRAP value of Sample (μM)
= (Change in absorbance of sample from 0 to 4 minute / Change in absorbance of standard
from 0 to 4 minute) X FRAP value of standard (1000 μM). I am confused with regard to Change in absorbance of standard from 0 to 4 minute. What should be the concentration of the standard with regard to the change in absorbance of standard from 0 to 4 minute?
I think you have to plot calibration curve of reference or standards so that you would be able to calculate frap in milli or micro mole equivalents
Can you please explain if we work with Trolox as a standrad than how is FRAP value of standard is calculted??
and we plot a graph for comparison of standrad with the extract both should be in same units i.e. mg/ml as extract could not be in moles
Than how can we compare it with FeSo4 which is in moles??
First of all, you should choose the way in which you are going to express your results.
For example, you could measure the absorbance change of a standard antioxidant sush us trolox, gallic acid, ascorbic acid or else you can use the IC50 value.
In my opinion, make a literature review about frap calculation in the sample you work on. There are plenty of published works.
Thanks for your inputs. Hopefully, I can proceed further with the suggestions that I have received from everyone.
Please find the attached of this review article on antioxidants
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