Hi Mariam,

Could you possibly post a chromatogram of the peaks you have troubles integrating? Also, it would be useful if you could post information on the software package you are using. This would allow us to help you integrate all your peaks properly and consistently.

Thank you!

Hi Marian,

Are you integrating the total ion count (TIC) or just a single mass of your FAME compound?

Alex

Peak height multiplied by the half peak width calculation peak area is to symmetry of chromatographic peak is seen as an isosceles triangle, the peak area is an approximate simple results. The actual peak area is A=h*σ（2π）-2

when the height is Y1/2=2.354σ, the area A‘ is： A'=h*y1/2=2.354σ

so A'/A = 2.354σ/h*σ（2π）-2 = 0.94

so  A = 1.06A'

Nowadays, you shouldn't have problems integrating the peaks with the software provided with the chromatograph and the detector. If you tell us your software, I'm sure we can help. Good luck!

Mariam Fares Alsharifi In the following video tutorial, I have discussed how to calculate area under the curve using Origin. Area under the curve signifies many physical and geometrical interpretations in Science. It’s a product of the quantities (functions) on the x and y axes. The video explains all the steps to be performed to calculate area under a specific curve. In the case you want to further ask about it, please do comment on the specific video, I'll respond to it shortly. I have provided the practice file here. Thanks

https://youtu.be/DMsBzGCNHQk