i have some data about a recombinant enzyme and i want to calculate my enzyme unite.
i have used the ellman method for enzyme activity test.
ODfirst minite= 0.35
ODseconde minite=0.8
reaction volume=0.2 ml (standard 96-plait, high 10.63 mm)
substrate concentration=1 mM
enzyme concentration= 0.5 mg/ml