Seyed mohammad Motevalli

14 Questions 21 Answers 0 Followers

Questions related from Seyed mohammad Motevalli

Why we need to do colony formation assay?

i am wondering why i have to do colony formation assay after MTT assay. there are two opinions which i learned from those people did that experiment. the first opinion is they do this assay to...

03 December 2016 441 4 View

According to Poly N-vinyl caprolactam, what will happen on Nitrogen in its ring in an acidic environment(for example in Lysosome)?

if we want to compare this polymer versus polyvinylpyrrolidone, which one is better to use as a thermosensitive polymer capable to transport a hydrophobic drug to a cell?

17 February 2016 480 3 View

How can I calculate the acetylcholinesterase enzyme units?

i have some data about a recombinant enzyme and i want to calculate my enzyme unite. i have used the ellman method for enzyme activity test. ODfirst minite= 0.35 ODseconde minite=0.8 reaction...

10 May 2015 2,721 3 View

How can I clean the procainamid-spharose 4B from contaminant proteins?

I have a home made procainamide sepharose 4B column that completely filled by contaminant proteins. Can I use of EDTA as cleaning agent? Is it possible the EDTA destroy the procainamide?

01 March 2015 9,950 4 View

Which buffer will interfere with DNS in different pH?

I am working on invertase assay and I want to study the enzyme pH profile, but I have problems with acidic pH in acetate buffer. The blank sample have same color with main sample. Can I use...

17 February 2015 6,093 1 View

Why does nanoparticle absorbance intensity increase after being treated by protein?

I treated the silver nanoparticle with acetylcholinestrase. data showed after 3 hours the maximum absorbance peak for nanoparticle-protein increased than blank one. in blank sample there is just...

16 December 2014 3,699 8 View

Is it possible to observe a the blue shift in UV-Vis spectroscopy after protein-nanoparticle incubation?

I am working on the interaction of silver nanoparticle in different size with acetylcholinestrase. i saw the blue shift in UV-Vis peak for treated nanoparticle with protein than blank one.

16 December 2014 7,542 6 View

How can concentration increment lead to size decrease in protein nanoparticle interaction?

I mixed protein with silver nanoparticle in different protein concentration. We showed increase of protein concentration caused decrease of size measured by DLS.

16 December 2014 557 13 View

How can I separate Ag ions from Ag nanoparticles?

There are some references showing that we can use cellophane as a semipermeable membrane and electrophoresis.

22 April 2014 5,537 4 View

Why can abiotic stress have more of an effect on cell death than biotic stress?

Biotic stress such as viral infection.

21 March 2014 2,756 6 View

Why surface to volume ratio in size and shape of nanoparticles leads to different effect on SPR diagram?

Decrease of surface to volume ratio in spherical nanoparticles led to red-shift in SPR diagram. But in same size nanoparticles, shape alteration, from sphere to cube, with respect to increase of...

25 January 2014 6,468 3 View

What are the differences between molecular dynamic simulation and energy minimization? How can i use the abalone software for calculation of energy?

I do not know if there are differences between calculated energy with MD simulation (such as Abalone) and energy minimization (for example with spdb viewer) and if there are, why?

17 September 2013 6,480 12 View

Could somebody advise on how to gain reproducible HPLC chromatograms(via C18 column) of HSA and AgNPs+HSA?

I think the proteins was trapped in column but i do not know is my idea true or not.

05 July 2013 2,483 1 View

Can anyone suggest a good tool to calculate the energy of each amino acids in a protein?

I think maybe I can calculate it based on one of the amino acids, such as glysine.

03 December 2012 3,869 1 View