I am mixing the drops(20 µl) of both reactants and measuring the change spectrophotometrically. Since the reaction is very fast, the change is observed immediately.
Would you please tell, based on what observation you are concluding " Since the reaction is very fast, the change is observed immediately."
I doubt if the reaction is fast without the use of a catalyst.The monmer formation will be slow and there will be sidereactions
Dear Dr. Shetty I do agree with the point made by you. Precisely, this was the reason why I asked the above question to Dr. Anil Mehr.
Actually, the reaction is monitored in a modified mass spectrometer. I could see the product forming (corresponding m/z values) within 2 seconds of addition of reactants.
Dr. Meher, I suppose you could take the help of HPLC. Record the peak areas at the retention time of the Schiff base so formed, at different time intervals after mixing the reactants. From the peak areas generated data, you could plot it against time to get the rate... Might help with some improvisation.
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