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01 June 2017 7,282 10 View

How can I calibrate a UV-Vis spectrophotometer?

I came to understand that I am only doing a check of absorbance instead of internal calibration. Anyone with useful information should please help.

07 July 2016 6,383 10 View

Can anyone provide me with a protocol for HPLC methods for assay of conbinational drugs like Artemether and Lumefantrine, Paracetamol and caffein?

Combination drugs HPLC assay methods

03 June 2016 7,025 1 View

I am in need of HPLC assay methods for common drugs aside those of BP and USP. Pls share if u have?

HPLC Assay guide. I am in need of HPLC assay methods for common drugs aside those of BP and USP. Pls share if u have?

29 May 2016 1,200 3 View

Please I am in need of SOP for pharmaceutical drugs. Anyone with useful material should please share? Not Bp or USP?

27 May 2016 4,438 2 View

Is high fat (lipid) intake the major causes of coronary heart disease, obesity and type 2 diabetes or high sugar (Sucrose = Glu + Fru) drinks?

Please go through these 3 references: 1. An excerpt from wikipedia: (https://en.wikipedia.org/wiki/Lipid) reads: "A few studies have suggested that total dietary fat intake is linked to an...

01 January 1970 5,268 36 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View