i want to check energy status of enterococcus faecalis in term of NADH/NAD,ATP/ADP and NADPH/NADP+ experimentally. Can anyone tell me non radiolabeled method for it.Any other suggestion to check the energy status are also welcomed.
You don't really need to extract NADH/NADPH to quantify them (It's the easier way, but not the most representative. Currently there are available fluorescence imaging methods for [NAD(P)H]/[NAD(P)+] determination.