How can I calculate association constant in fluorescence by substitution of fluorescence intensity?

Jubyeong Chae @Jubyeong_Chae

07 July 2018 0 3K Report

Hi,

I want to calculate association constant through following method

Talanta

Volume 41, Issue 4, April 1994, Pages 599-609

However, I don't understand why I-I0 could be used.

Could anyone explain about this equation in more detail??

Thanks

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