How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...

04 February 2021 6,580 2 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

Where can I find these two papers?

14 January 2021 1,572 3 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

What is volume of gas sample to be injected into GC for analysis?

I'm currently working on a batch type gas phase photocatalytic reaction. For product analysis, i draw the gas samples from the reactor using a gas tight syringe and then inject directly into the...

22 December 2020 6,850 2 View

What is the best column temperature program to analyze BTEX concentration in dichloromethane extractant using GC-FID method with hydrogen carrier gas?

I tried the temperature program below to draw my calibration curve using splitless injection but the peaks I got were so small and broad in the concentration range of 5 to 50 ppm. I was wondering...

19 November 2020 4,531 3 View

What are the best solvents for clean-up after ultrasonication extraction of PAHs?

I have done Ultrasonication extraction of PAHs using hexane:acetone. What is/are the best solvents to use for silica clean-up before GC-MS analysis?

17 November 2020 8,483 3 View

How do I reduce a GC-ECD high signal?

Hello, In the last several months, I've noticed that the signal in ECD in ambient temperature has increased a little, from 15mV it has reached 28-30mV. During the method's setting up, the signal...

13 November 2020 241 4 View

In online GC-FID/TCD connect to flow reactor, why I am getting instable peak area in FID in every injection under same conditions?

We have online GC-FID and GC-TCD (Mod: 2014) connected with flow reactor system. This GC-FID/TCD is connected to a gas line coming from flow reactor system which I used for cascade reaction. In...

11 November 2020 4,999 6 View

What is needed for Olive Oil Lipid Extraction?

I am doing my thesis and would like to extract triglycerides from Olives or Olive Oil. I will be analysing the amount of triglycerides that are present in the olives or Olive Oil I will be...

01 November 2020 8,314 5 View

GC COLUMN SELECTION, Can Bpx70 column be used for volatile fatty acids in rumen fluid in aqueous state on GC-FID?

I want to analyze rumen fluid samples in aqueous state for volatile fatty acids on a GC-FID. We only have a Bpx70 column. Can this column be use?

24 September 2020 7,797 4 View

What kind of compound is named: C(Si)(C)(C)O(Si)(O)(Si)(C)(C) et cetera?

Hi. I'm working on methyl linoleate oxidation products, analyzed by GC-MS. When trying to identify a peak, my libraries give a perfect match with a compound with a crazy name that goes as follow:...

23 September 2020 9,339 2 View