How can I avoid genomic DNA contamination in RNA extraction using Trizol method?

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Sami Ullah

Dear Qari,

The first thing that you may practice is that to isolate the RNA in sterile conditions. secondly, use DNase I to remove DNA contamination. I can give you a good protocol if you can tell me from which source you want to isolate RNA.

Qari Muhammad Imran

Dear Sami Ullah.

i am extracting from Plant source (Arabidopsis thaliana).

Have a look here. But I would do suggest that use DNases

thanks alot Hall that helped alot