I want to analyse my sample (small amount) by DLS at increasing temperatures. But air bubbles start to form around 65 °C and disturb the signal. Does anyone have a solution?
You should degas your buffer. Place the buffer in an open container in a desiccator. Gently pull a vacuum until you see bubbles appear. Be careful not to pull too strong a vacuum or you will boil your solution. You may need to gently tap the desiccator on the table top to get the bubbles to dislodge from the sides of your buffer container. Then gently release the vacuum. Keep your buffer in a tightly closed container and avoid shaking or vigorously stirring it to prevent gas from dissolving back in to the solution. Also, keep the amount of air space above the solution to a minimum (don't put 100 ml into a 500 ml bottle).
Thanks to both of you for quick answers.
The problem with David's solution is that I have only a small volume of my sample (about 100 µL). Your degasing technique does not seem appropriate for small volumes...
The oil might be a good solution, I'll try this.
Degassing does work for reasonably small volumes. I have done it with 100 microliters in an Eppendorf tube without a problem.
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