Hello Friends,
I want to see whether a particular stem cell population can differentiate in vivo (into mice or rat model) into functional neurons or not, if injected in brain.
In vivo imaging or sacrifice of animal and then doing IHC ?
I am confused. Please do give your substantial inputs and any new ideas whatever click into your minds.
Thanks !
Can't imagine in vivo imaging will help so much. You should do IHC for neuronal markers - which won't tell you if they are functional - just that they exhibit those markers. for functional studies, you might need vibratome sections for electrophysiology, or use minimacs separation for ex vivo functional assays (but not sure which one)
DEAR AJAY, MAKE SERIAL STUDY OF DEVELOPMENT OF NEURAL TISSUE AND STUDY THE EXPRESSION OF SP. Protein or its SP. Marker in dev. Stage specific manner.
@ Rajneesh K Sharma, Thank you so much Sir. This is a side which I ignored, so I may take the same into consideration.
Can you please elaborate what is SP (did you mean specific proteins) ?
Regards!
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