How to Start My Research Regarding Microgrid ?

Hello there. I am new to research and my research area is about "Energy Management System in Microgrid". I had already studied some research papers to have a better understanding on that...

01 June 2020 1,046 6 View

How to separate phospholipids from betaine lipids using silica chromatography?

I tried separating betaine lipid from phospholipid and galactolipid from my 2D TLC plate but to no avail. Betaine lipid was found to overlap with some galactolipids under the condition of:- 1D:...

25 March 2020 641 3 View

How can I find the XRD data of RuI3?

I fabricated RuI3 with CVT method, but I am not sure that this material is RuI3 or other Ru compounds. I checked the reference that one paper (published in 1968) had analysed the structure of RuI3...

04 June 2019 1,681 6 View

How to calculate the amount of nanocellulose in a solution accurately?

After preparing nanocellulose, how to calculate amount of nanocellulose inside solution? I would like to separate it into few portion in solution form for adsorption test.

12 February 2017 8,698 1 View

Embryo melanization?

I study on an endemic species of biting midge (Forcipomyia taiwana) in Taiwan. This is a small (ca. 1.4 mm), slender biting midge that is present island-wide in urban and suburban habitats in...

10 November 2013 1,438 2 View

Is it possible that pH of wastewater can reach 8.5 even 9.0 from 4.5 in anaerobic reactor?

Anaerobic reactor.

06 November 2013 2,415 7 View

Is multi-homing common in the market of razor and blade?

In the market of razor and blade, some consumers own multiple razors like owning Gillette and Schick razors at the same time. Consumers then have to decide which razor to use for shaving when they...

01 January 1970 8,200 3 View

Why are the razor priced low and the blade priced high?

In hardware and software markets, the hardware is usually priced high, and the software is relatively cheaper. However, in some tied markets like razor and blades, printer and ink cartridges,...

01 January 1970 3,802 3 View

Is it possible to have active lupus but with negative ANA and anti-dsDNA?

I have a female lupus patient, she had been experienced several times flare and had received treatment of prednisone, CTX, MMF, or RTX one after another. She present as pancytopenia and pulmonary...

01 January 1970 244 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View