I'm using Proteome Discoverer 3.0 to perform database searching for LFQ DDA MS data. The input file is .raw file. the output data is scaled or normalized abdunence. What I want to do is get raw abdunence from PD, what should I do?
We are using pMSCV for transit expression of a protein.: 1. The gene was cloned in (between XhoI/EcoRI). 2. Transfection with lipofectmin 3000 to 293t cell. 3. After 48 hrs, GFP can be...
04 April 2024 8,350 4 View
There are many spots on the tomato fruits, the size of a pinhole in the middle, and sometimes there is a white ring outside. Please help us and identify this pathogen. Thanks a lot!
01 April 2024 4,895 2 View
microbial communities
11 January 2024 8,106 0 View
Did there exists a dataset which full of Android API sequence(dynamic best)?,I find some, but they are all apk file, if you know please inform me that, thanks!
06 December 2023 3,293 1 View
JEOL 6300FS ZEP 4000rpm baked 180℃ 5min dose 250 CD 20 Pitch 102
06 December 2023 8,872 0 View
I have expressed an Fc fusion protein in 293F cells, and the expression in the supernatant was so good, but when I purified it with Protein G agarose and Protein A agarose, it cannot be eluted. I...
05 November 2023 9,562 1 View
I have a set of PV, wind and load demand data which I used for microgrid energy management system. I wanted to add noise to my data to test the robustness of my system. However, I realized that my...
08 October 2023 9,988 0 View
I am studying geotechnical properties of clay-like pumice containg clay mineral halloysite. But I met one problem, for example, I do not konw how to make the specimen of soil to observe its...
05 September 2023 5,239 4 View
I'm new in NGS data analysis. Recently, we sequenced a hairpin library (~1000 shRNA) for quality and We already got the fastq file from illumna. We need to get information from this fastq file:...
11 June 2023 1,148 3 View
Elon Musk is continuously talking about replacing low-voltage fuse and did not involve low-voltage fuse in Model3. However, high-voltage fuse remained. I wonder the reason why.
14 April 2023 2,398 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View