How can I analyze samples that were run in different Nanostring miRNA codesets without a calibrator sample?

11 November 2019 1 2K Report

Hi all,

We have samples that were run in different codesets (v.2.0, v.2.1, v3.0) and now we want to analyze them together. What would be the best way to do the normalization?

We talked to the Nanostring group, and they said that we should have a calibrator sample, meaning one single sample that was run in the 3 different codesets.

We don't have that (these samples were from different projects and run in different years). Is there any other way to normalize this data without a Callibrator sample?

Arnaud Beurdeley

Hi Bruna,

For this point I think you are facing an issue... as nano string told you, you need to get one sample at least, to run a bridging study...

Is there any RNA sample left from your previous analyzes (V1, V2...) to run on the miRNA V3 ?

Best

Arnaud

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