I want to compare two below images for example.i want to know, in which image exist more collagen fiber? for this goal, i need to know, how can use Image j software, step by step, because i never use this software.
thank you for your attention.
Guillaume Gauchotte
Hi Saeed
If possible for you to perform another staining, I think that a staining with Sirius red would be more reliable to quantify collagen by image analysis.
If you have Adobe Photoshop, it is very quick and easy to evaluate the collagen area with the "color range selection tool" (then divide the number of selectid pixel by the total number of pixels of your picture).
Best
Guillaume
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