How can I amplify the intergenic region (IGR) of the tospo virus?

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Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

CUDA Out Of Memory (OOM) error while using GPU?

I'm training a neural network (E3D-LSTM) for spatio temporal video prediction. It's quite a big network that has millions of parameters. The model is training perfectly when using my 12 CPU cores,...

25 December 2020 1,229 3 View

Which area i should focused in FSW of Al-Steel with interlayer?

Can anyone give me suggestion about topic to carry my research? As i see there is number of publication in this area.so i am not getting that which objective i should keep for my research.

04 September 2020 2,286 2 View

Comsol electromagnetic simulation. How to give a mesh for certain micrometer when my tool is in millimeter. The current density is so small. Pls help?

Hi, I am trying to perform mesh convergence and I found out there is no stable temperature value I obtained. So, I calculated the skin depth and it was around 0.00011mm. It is so small in...

24 August 2020 9,060 2 View

Which option (SFA-Cross Or DEA-DMU) is best to measure the individual efficiency (for a sample size of 450 farmers)?

I am working on a set of data, collected from a field survey. I am looking forward to measure the the level of technical efficiency of type of irrigation channels (Solar pump set/ Diesel pump set...

06 August 2020 2,510 3 View

How to increase accuracy in Speech emotions recognition ?

In speech based emotion recognition, for individual artists almost get 90 percentage of accuracy using neural network, but accuracy is decreased up to 70 percentage for entire speech corpus. How...

19 July 2020 4,900 1 View

What are the different methods of estimating snow melt contribution in runoff?

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16 July 2020 6,415 3 View

How to model in COMSOL (Induction heating) . A current applied for 600A for 10 min and continue then 100A for next 5 min. Can someone help me please?

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10 April 2020 5,565 12 View

I'm having a hard time to get the UV-vis spectra of acetone. What may be the reason?

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11 November 2019 9,477 5 View

Thermal topology optimization run ends with error due to memory. How to swap memory?

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06 November 2019 8,994 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

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02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Amanda O'Donnell

Have you tried lowering extension temperature to 60oC?

Himanshu Shekhar

Check this map may be it help you to design a proper primer.

Vishal Gurav

Thanks Amanda and Himashu for ur kind reply, I'll definitely try to follow ur suggestions.

but Earlier i've used different specific primers with various annealing temperatures. I've also used Pfu DNA polymerase but positive results r not yet found.

Lieven Gevaert

better use a pfu taq mix and or hot start pfu

http://www.gentaurpub.com/Tospovirus/VG9zcG92aXJ1cw==.html