Methanopyrus kandleri is one of the methanogenic bacteria living in deep sea hyperthermal and hyper pressure cultivation condition. It seems production of methane by aid of these bacteria could be potentially economical especially in the lands with warm and high temperature solar radiations like deserts but to get optimum growth condition high pressure factor is important too and to get the highest gas production pressure condition should be the same as deep sea. By using solar heat and radiation to ensure temperature factor, what alternative would you suggest to overcome the problem of reaching desired environmental cultivation pressure?
see the attached paper whether it answer your question partly if not fully
dear Sohail I already have read this article. problem is about the cost and benefits of procedure of methan production. there are places with high solar radiation and temperature so you can provide heat by sun but to get the maximum cultivation output and bacterial life cycle 0.4Mpa pressure is needed. how this environmental pressure can be provided without any other cost of facilities?
maybe it depends on design of culture batch
dear Annett,
M. kandleri strain 116 is piezophilic, optimally growing at 105°C at hydrostatic pressures between 20 and 30 MPa.
Takai et al in the article "Cell proliferation at 122°C and isotopically heavy CH4
production by a hyperthermophilic methanogen under
high-pressure cultivation" used the protocol as"The cultivation syringe was pressurized in the distilled- and deionized-water filling in a titanium hydrostatic pressure vessel (Teramex),and then the vessel was incubated at a given temperature up to 110°C in atemperature-controlled dry oven (Fig. S1). If the incubation temperature was
over 110°C, a stainless steel, high-temperature, high-pressure reactor (up to
500°C and 60 MPa) (Nitto Ko-atsu) was used instead of a small titanium
pressure vessel."
i was wondering if this protocol is applicable to industrial establishment but i'm thinking about designing solar system to provide high temperature and about pressure for optimum growth rate and methan production ( for example if we want to generalize this method for home usage) to make it more economical
Methanopyrus kandleri is normally found associated with volcanic vents, It reduces carbon dioxide with hydrogen which is unlike other methanogens which generate methane primarily from formate. in this case under the sea. It is an archea type of prokaryote. You may succeed in generating methane in the lab but rule out all possibility of it being used for commercial purposes.
i'm not expert in microbiology but i was wondering about its commercialization coz its potentially noticeable.. what is the biggest problem about driving large scale cultivation?
Dear Hawa,
if you're interested in culturing thermophilic methanogenic archaea, I would suggest to have a look in strains that are not barophilic/barotolerant at the same time because maintaining high pressure will be a big problem for any large-scale applications that you have in mind. I just did a very brief literature search and it appears that quite a bit has been published about thermophilic methanogens in hot springs - this might be more analogous to your needs than a deep sea hydrothermal vent.
Growing anaerobics out of the sea you need enclosed and airtight container. Use a recycled plastic barrel and expose it under the sun. Methane produced will create pressure in the barrel. If the methanogens you are using can only tolerate to a certain pressure, just install a barometer and controlled the amount methane in the barrel by using a valve. If a hyperpressure needed prior to cultivation, pumped in inert gas until desired pressure is achieved. Alternatively you can save cost in producing methane at normal pressure by cultivating methanogens from cow dung/chicken manure/palm oil mill effluent and produce methane using a biodigester system.
http://biogasdigesterdiy.blogspot.com/
Terrestrial biotopes of hyperthermophiles are mainly sulfur-containing solfataric
fields.Terrestrial biotopes of hyperthermophiles are mainly sulfur-containing solfataric
fields. These are also found in superheated walls of deep-sea hydrothermal vents, Methanogens can be cultivated in natural media with exposure of sunlight, but it needs pressure, temperature scaling with acidic pH. The protein molecules in the hyperthermophiles exhibit hyperthermostability Hyperthermostable proteins are often important, for chemical reactions proceed faster at high temperatures. To get optimum growth condition high pressure factor is important . It should be maintained muck like deep sea.
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