Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How is mathematical giftedness supported in different countries?

I hope for a global overview on mathematical giftedness and its support in school and/or on an extracurricular level. What programmes/opportunities are offered?

08 July 2020 7,124 4 View

GROMACS - how to solve problem coming out during a creation of new Force Field?

Following Gromacs Manual and other suggestions from different topics I create a molecule with Avogadro, I decided to employ oplsaa ff (I copied it in a local directory giving a new name) and I...

25 March 2020 4,438 3 View

How to create a fictitious atom in shelx?

Dear all, I am dealing with some crystal structure in which it is possible to replace Br- anions with Cl- anions. I am trying the refinement by setting the occupancy factor according to the molar...

24 February 2020 4,122 2 View

Trying to run RT-PCR for PDCD4 gene in human serum, can't get any values. Checked primers and they seem okay, any suggestions?

We are having difficulty detecting PDCD4 in serum. We have ordered an ELISA to attempt this, but have not tried it yet. Afraid something is wrong with serum.

08 January 2018 4,637 3 View

How to check for primer dimers?

I am having problems with high efficiency of my primers and one reason could be the formation of primer dimers. I dont see any big peak on the melt curve, but I dont know if this increased peak is...

19 September 2017 7,270 4 View

Checking RNA integrity for qPCR with electrophoresis but it does not show 28S/18S ~ 2. Should I extract again?

I have run RNA samples in 1% agarose gel but I cannot visually see a ratio 28S/18S ~ 2. What I might be doing wrong? Also, the samples marked with numbers, should I still consider them for qPCR?

12 September 2017 6,958 6 View

Is there any phenotype database for Streptomyces?

I'm identifying my Streptomyces isolates at the specie level. I already performed all the phenotype / chemotaxonomic tests, and constructed a phylogenetic tree. However, I didn't find the...

16 April 2017 1,713 3 View

Griess Reagent usage in fibroblast cell culture?

Hello, I am trying to measure NO in cell culture (fibroblast cells). Using Griess reagent I try to follow the paper from Wang et al., 1996 which gives a basic protocol that does not differ from...

07 October 2016 8,597 1 View

What test can I use to investigate the correlation between Ratio scales and Ordinal numbers on SPSS ?

05 October 2016 4,612 9 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

How can I calculate teh crystallinity degree of graphene oxide?

I had treat my XRD diffraction pattern by Highscore to calculate the crystallinity degree Xc and I have the formula but I dont no the 2Theta of Ac and Aa to calculate Xc. Is it like polymers...

10 February 2021 6,305 5 View

Could any one help me with mechanical properties of CFRP(carbon fiber reinforced polymers)?

I am modeling CFRP laminated a concrete column in Abaqus And I need Elastic and plastic properties of CFRP

09 February 2021 4,930 5 View

Dear colleagues. How can we implement the kinetics of the polymerization of aromatic amines in easier and simpler ways?

Dear colleagues. How can we implement the kinetics of the polymerization of aromatic amines in easier and simpler ways? Are there simpler methods for Nano polymers (other than those obtained with...

09 February 2021 5,783 3 View

How to calculate enthalpy of formation of Polymers taking into account the terminal end groups?

I am trying to find methods for theoretical calculation of enthalpy of formation of Polymers. till now I have found the oligomeric method (Gaussian) which uses the extrapolation of results from...

05 February 2021 9,707 4 View

What are advantages of NELF model?

I am trying to simulate permeation of gases in polymers. For this reson normally i use the clasic models like dual sorption model but newly i foun dthe non equilibrium lattice fluid model. It...

03 February 2021 1,203 3 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View