How can DNA sequencing help in fighting or reducing rate of bacteria infection?

Peter Ayodeji Idowu @Peter_Idowu4

05 May 2018 0 7K Report

Incessant use of antibiotics, resistant to drugs

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What are the habits or capacity that supervisors looks into in order to accept a new student into their research group?

Research output, Ethics, Statistical exposure.

08 September 2019 10,061 4 View

What could be reasons for a manuscript to be extremely delayed for months after the required actual months for reviewing process?

And in such case? Is it advisable to withdraw such manuscript? For example a journal has 3 months for review process, but such manuscript has stayed for 6+ months. Dear researcher, what will...

10 November 2018 2,949 17 View

What are reasons that can be given by editors in rejecting a well written and good manuscript?

Rejection, Publications.

05 June 2018 808 1 View

Dear researchers, Please is there any free or less expensive english editing software that can be used for Grammar improvement?

Note: I am not asking for plagiarism checker. But English editing software or applications. Thanks

05 June 2018 1,684 0 View

Dear researchers, Please can you assist with list of free journals on animal nutrition? Also can value of a journal be determined by its charges?

Considering that it is peer review, high impact factor? high h-index,

04 May 2018 1,081 3 View

What are the factors to consider in publishing in a credible or reputable journals?

Publication duration for reputable journal

03 April 2018 3,189 4 View

Is it possible for two identical twins to have SAME DNA sequence? disregarding DNA mutation or other environmental influence?

DNA sequencing

03 April 2018 6,904 21 View

Dear researchers, Which gender mostly participate in poultry production? If women, please give reasons why it is so?

Poultry production, Demographic reports, Gender and Poultry

03 April 2018 4,570 0 View

Can anyone help with software to use to analyse questionnaire deeply apart from SPSS and STATA. Especially natural science based software?

Please your answer will be widely appreciated.

01 February 2018 7,153 3 View

What is the meat colour of stressed beef before slaughtering?

Meat Science

08 September 2017 4,993 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View