How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

I am finding a lot of cellular debris in the culture flask? What are the reason behind it?

I am facing a problem that cellular debris observed in my SHSY5Y cells. I have seeded the cells in DMEM media with 10% FBS in T-25/ 60mm dish (antibiotic used PEN-Strepand gentamycin). I have also...

17 January 2021 3,074 3 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View

What is the chromatographic technique used for the Gentamicin quantification?

HI everyone, I want to quantify the gentamicin antibiotic presenting in the culture medium. Can You anybody please suggest me suitable chromatographic technique for that? Please suggest me the...

16 December 2020 9,823 3 View

Are my Small Cell Lung Cancer (SCLC) showing normal confluence and morphology?

Hello, I am new to cancer research and I am trying to optimize my small cell lung cancer cell protocol. I purchased a cell line from ATCC (H1105) and I have been growing the cells in RPMI 1640...

11 October 2020 5,670 6 View

How can you do biotynilation of antibodies in presence of gentamicin?

For some time we have used biotinylated antibodies for ELISA. Usually I use biotin N-hydroxysuccinimide ester to link biotin to antibodies (and I have used different carbodiimides for other...

28 July 2020 8,806 3 View

Anyone knows what causes eye lid problem in Siberian hamster?

Several months ago we observed some strange changes around eyes of several individual Siberian hamsters (Phodopus sungorus) in our colony (outbred). I attach pictures. It looks like an...

17 July 2020 9,941 3 View

Why does my H9 IPSC die reasonless after 80% confulency?

Dear All, I am working with thermo fisher H9 cell line, human induced pluripotent stem cell. I made a mutation on them with crispr cas-9 I obtain single colonies and I propagate my clones. I...

13 June 2020 9,046 3 View

Unable to select rpsL-mediated streptomycin resistance?

I'm trying to isolate spontaneous streptomycin resistant clones of Neisseria meningitidis. I have done this before, and I usually pick up mutations in rpsL (K43R or K88R) in the standard strain we...

12 February 2020 5,372 1 View