Dears,

I'm using the UV/vis method to determine water-soluble chrome in cement as follows:

I take particulate mass from dichromate to make a stock solution then take various volumes of aliquots into a 50 ml volumetric flask to set up a calibration curve.

for measuring the sample, I take a 25 g sample and 25 ml of water then filter and take 5 ml of filtrate into a 50 ml volumetric flask and dilute to mark with water after adding a color indicator.

my inquiry why don't I consider 50 ml dilution of aliquoted sample in the calculation equation ? and if I deviate from the method and make a dilution of the aliquoted sample 100 ml instead of 50 ml what does the formal calculation equation become?

#analytical Chemistry