For applying to a fabricated biogas reactor and oil screw expeller, the main material to produce TG/TAG (elot) should be a high content of glucose.
A dry sediment of tapioca starch wastewater suspended solid consists of 11.6 to 12.9% amylose. Amylose is a long chained linear polymer of α 1-4 linked glucose molecules. Amylose tends to form a helical structure decreasing the ability of amylase and other enzymes to degrade the molecule. Amylopectin is a branched polymer of α 1-4 linked glucose molecules with α 1-6 linked branch points11. The conversion of starch into glucose in the batch is done through two stages, namely liquefaction and saccharification. Liquefaction stage is done at atmospheric pressure, a temperature of 95-105 oC, and pH 6-6.5 for as long as 1-2 h. Saccharification stage was performed at atmospheric pressure and a temperature of 55-60 oC for 8 h to get a dextrose equivalent (DE) is quite high12. This study was not conducted on liquefaction and saccharification. A process undertaken was only medium sterilization using autoclave at a temperature of 121 oC and atmospheric pressure for 15 min. The sterilization process only happens a gelatinization process. In this study, starch hydrolysis process has not occurred completely. Consequently, only a few lipid (TGs/TAGs) was obtained at the end of process production of lipid. This might be a reason, why lipid yield was low.
I hereby respond to your answer for the sake of readers/public who might have been confused. You seem not to understand my question nor appear to have the principle of exergy, which is second law of thermodynamics. Your response is far from the knowledge of exergy Therefore, your reply to my question is wrong. kindly consult journal entitled "Cynthia Ofori-Boateng ⇑, Lee Keat Teong, Lim JitKang. Comparative exergy analyses of Jatropha curcas oil extraction methods: Solvent and mechanical extraction processes. Energy Conversion and Management 55 (2012) 164–171". to understand the principle of exergy