Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

Why build a Leaning Tower of Lire?

What are the lessons to be learned from building a leaning tower of Lire?

03 January 2021 7,364 4 View

What are some of the applications of transparent wood?

Are there any particular uses for transparent wood other than visual appeal? The transparent wood is really cool to see, but what are the practical uses? Please provide examples. Happy New Year!

31 December 2020 2,479 4 View

Can light waves induce electricity through induction?

Can light waves induce electricity? I am unclear on this matter. Thank you.

30 December 2020 7,974 3 View

What are the benefits of applying Blockchain within any industry?

Is blockchain only for cryptocurrency? Thanks in advance.

28 November 2020 5,086 11 View

Is my Chi-square test valid?

18 November 2020 3,033 2 View

Confused primer dimer or something else?

Hi everyone, i recently did a qPCR to check the best annealing temperature for my primers. So i ran two of my primers on annealing temperature 59C with a melting curve. The melting curve shows...

17 November 2020 1,913 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View