What test should I use?

15 February 2021 911 5 View

Why am I not getting colonies using the Agilent QuikChange II kit?

Hello everyone, I hope you are very well. I am performing site-directed mutations with the QuikChange II kit. My first ones have a length of 50bp and a Tm of between 75 and 81 approximately. I...

09 November 2020 242 2 View

Repurposing a published image for a thesis - permission requiered?

I would like to use a crystal structure of the heme group from PDB for my thesis, for the introductory topic of cofactors (Figure 2, 10.1111/j.1432-1033.2004.04411.x ). The published figure has...

19 August 2020 1,798 3 View

How to convert .txt into plink files (ped/map or bed/bim/fam)?

I have a huge oncoarray_genotype_XXXX.txt file with 11.7 GB size. I would like to convert this file into adequate files for analyzing in plink software. However, I could not find how to conduct...

08 July 2020 9,302 2 View

Using RPMI vitamin solution for Bacteria growth?

I am searching for a solution containing both essential and non-essential amino acids. Mammalian cell culture seems to use these quite often, I would like to know if someone has experience using...

16 March 2020 7,696 3 View

Flow cytometry multicolor panel builder software tool that scans all commercial antibody databases?

Does anyone know of a web app or software for designing polychromatic flow cytometric panels that's able to scan all commercially available primary conjugated flow cytometry antibodies (i.e. from...

13 December 2019 7,047 4 View

What concentration do you use to elute a NiNTA resin?

The protocol of Thermo Scientific NiNTA resin (cat. 88221) for purification of proteins containing a hexahistidine tag use an elution buffer containing 250 mM imidazole. For fun after this elution...

01 August 2019 4,271 3 View

Dehydrogenase enzyme activity: would you take a look at my data?

My experiment involve a dehydrogenase and I would like to identify the optimal substrate. For this an assay was established based on the use of DCPIP as cofactor, which when reduced the absorbance...

11 January 2019 2,476 5 View

Is one gRNA enough to delete a region by CRISPR/Cas9, whose HRs are ~1kb apart?

Dear all, Me and a colleague have come across the question whether a single guide RNA is enough to sufficiently delete e region (let's say a complete KO), for which the two homologous regions are...

26 September 2018 5,318 3 View

Can I use RNA protect for RNA bacterial extraction inoculated on food matrix, and after that, dilute it?

Hi people! I would like to ask whether anyone of you have extracted RNA from bacteria on a food matrix. I have to inoculate bacteria on nuts, and after my processing tests, I need to extract RNA...

11 September 2018 7,343 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View