I am extracting protein using TCA and quantifying with BCA assay, I get low values (
Dear Dr Eseiwi Obaseki,
I think the protocol found in this article (attached), may actually help you to perform protein extraction with TCA ppt for high yeild.
Best wishes,
Sabri
Thank you, Mohamed. It seems the attached is specific for plant tissues.
It's my pleasure dear Dr Eseiwi Obaseki.
I wish you all the best in your research.
Good day all, i encountered a strange problem with gromacs when restarting my simulation. i ran a 100ns simulation and then created a new tpr file named newproduction.tpr to extend my simulation...
04 October 2023 968 4 View
In the current climes of digital transformation, cloud adoption, big data and Artificial Intelligence, etc., there is a need for every forward thinking organization to have in place a framework or...
05 October 2020 8,692 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
31 July 2024 8,666 3 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
how can I extract my bibliography from researchgate ?
28 July 2024 6,737 1 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View