15 November 2022 1 5K Report

I need help plotting connectedness network graphs for my data

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What are the possible methods to prevent the digestion of antibodies by proteases during affinity chromatography?

Aside from inhibiting protease activity (not effectively working), I read some papers about doing modifications to the antibodies: using unnatural amino Acids using mPEG But, are there better...

15 February 2023 4,268 5 View

Difference between the Beam and Bar element in ANSYS ?

Hello everyone, When I am doing the dynamic analysis for a frame using ANSYS, I found that when I use bar element or beam with only one division, I got approximately the same result, but I...

14 May 2021 6,328 2 View

Metagenomic sequencing of Bacterial DNA from Clinical Swab?

Hi RG community, We are running WG metagenomics sequencing as a diagnostic tool and would like to get rid of host "Chicken" DNA to be able to increase the depth of sequenced bacterial DNA "for...

01 December 2020 369 6 View

Primer-template mismatch?

Dear all, May I get some guidance or help on: 1. How many primer-template mismatches would lead to PCR failure? 2. I know they 3'end is more critical but does any mismatch at the 5'end would...

11 May 2020 8,950 0 View

QPCR application on Killed vaccines?

Dear All, Is it possible to perform qPCR on Killed viral or bacterial vaccines? I read that it depends on the method of inactivation because some of the inactivation methods degrade the NA. Does...

23 January 2020 1,832 5 View

What happened to this?

Where i am from we rely heavily on water tanks for houses and we monitor water level by going on the roof multiple times because you don't want to overflood it but also it sometimes come at late...

07 November 2019 9,210 0 View

I am implementing a routing protocol in MANET and I need to send a beacon to collect data a bout the current network before starting sending in ns2 ?

I am implementing a routing protocol in MANET and I need to send a beacon to collect data a bout the current network before starting sending a route request could you please help me the data i...

30 September 2019 3,067 5 View

How To use gray level images (Ultrasound) in CNN as they have 1 channel and CNN use 3 channels (RGB) ?

How can i customize my Convolution Neural Network (CNN) to deal with gray images (2D ultrasound) as the input layer is something like (3, 256, 256) the 3 represents the R, G and B channel in...

22 June 2018 9,158 8 View

Checking H2 by TCD and He as carrier gas ?

How can i check H2 10 % in argon using TCD and helium as carrier gas ?. the GC is Agilent HP-7890B Mol Sieve column .

24 April 2018 8,696 0 View

What the bet combination GC-PHID ?

what the bet combination GC-PHID ? to check high purity argon , nitroen , helium .

29 November 2017 2,510 0 View

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Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

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Is there a name for this type of function?

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