We are having difficulties in writing MATLAB code for microscopic image feature extraction and processing. Does anyone work in writing programs for biological research?
I'd recommend that you use an application to figure out exactly what kind of analysis you need to do, and then use scripting to automate it.
For graphical applications, try cellProfilier, Fiji/ImageJ, and Ilastik. Once you can find a workflow that suits you, those programs may just be able to automate the workflow directly. Most of them are designed to fit this design pattern- you come up with analysis, they can support running it in headless mode.
If the graphical applications don't give you the analysis you want and you really need to crack open the image processing toolbox, I'd recommend scikit-image in python over matlab. That's just me.
In any case, come up with your workflow first. What do you want to do with enzyme research? What are your images like? What information do you need to extract? Answer these and plan out your task before you write a single line of code. A great start is to email an image mailing list like imageJ or scikitimage and say "here's my image, here's what I'm trying to extract, what are your suggestions" to get started.
There are whole lot of researchers working on image feature extractions in medical images (usually MRI,fMRI, SEM, and other scans, but I'm guessing since it's a microscopic image this is relevant to you).
A very good starting point would be to look at research done at (SCI): http://sci.utah.edu/research/image-analysis.html?cat=ImgAnal
in the filed of image analysis.
With regard to Matlab code, mathworks forums would have many user-donated codes for feature extraction (I'm sure Matlab's image processing toolbox has many built in feature detectors)
Dear Karthikumar Sankar
you can use wavelet transform curvelet transform for feature extraction
dwt2 in matlab
Regards
- Badges
- Science topic