I am looking for a host at University of Bonn in my line of research for a short research stay. My line of research is in plant-microbe interactions.
Can anybody provide insight on why a sample of SYBR green mastermix, straight from the bottle, never amplified, never opened, would provide a band of ~900bp on an agarose gel? This was discovered...
29 August 2021 9,597 3 View
I'm trying to get the FoldX 4.0 plugin working in Yasara View (the latest version, 21.6.2) on Windows 10. I've extracted all associated files, including foldx.exe and rotabase.txt into Yasara's...
01 August 2021 1,128 1 View
I am labelling myristoylated proteins in cells and performing a click chemistry reaction to add the fluorescent TAMRA-alkyne to visualise via in-gel fluorescence. In a negative control sample...
28 March 2021 1,104 1 View
I am looking for a chemical aromatase inhibitor that isn't also biologically active in other ways (or as minimally as possible). Any suggestions? Ideally, should be able to be ingested without...
05 February 2021 8,384 2 View
Hi all! I want to use acceptor photobleaching FRET to look at a potential interaction between two proteins of interest. We have a Leica SP8 in my lab that I have used to try and get an idea if...
09 March 2020 2,530 2 View
I am researching two isoforms of an endolysosomal ion channel. I have found that when one is knocked down with siRNA, the expression of the other increases and vice versa. I cannot see this being...
20 August 2019 6,951 2 View
Hello, I am trying to figure out the protein product of a chromosomal translocation. I have the chromosome and location (base number) of the breakpoint. I would like to know if the breakpoint is...
18 May 2019 6,993 6 View
I want to analyse the differences in male and female responses to a questionnaire assessing perceptions of gender equality. The questionnaire uses likert scale data only. Which statistical test do...
21 March 2019 9,698 5 View
I am currently working on isolating and screening rhizobacteria. One of the properties am concerned about is nitrogen fixation ability and so I am growing the bacteria in nitrogen free media....
14 September 2018 4,512 15 View
I am working on an anti-idiotypic ELISA for human clinical trials and would like to know if human serum is diluted in these assays or used neat. My ab of interest is an IgG4.
01 August 2018 4,836 4 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View
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24 July 2024 1,559 1 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.
14 July 2024 3,120 7 View
To combat environmental contaminants, plant tissue culture labs are fumigated. Would vapourised hydrogen peoxide have a negative effect on the tissue cultured plants in jars?
10 July 2024 8,815 1 View
In plant breeding research, line x tester mating designs are often used to assess gene action for various traits. Some studies suggest that the simultaneous significant mean squares for lines,...
30 June 2024 4,877 2 View