Hormone levels in male mice ?

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Could anyone provide me a spreadsheet to present the composition of sulphides in diagrams?

10 April 2024 6,290 0 View

How to calculate the Mean normal partial thromboplastin time ?

Is it like MNPT (geometrical mean of at least 20 healthy subjects) ? or it's the arythmetical mean ? can a pool of healthy subjects' plasma be considered as the reference APTT for calculating APTT...

01 April 2024 1,846 0 View

What are teachers' motivation strategies?

This research aims to investigate the relationship between teachers' intrinsic motivation strategies and EFL students' outcomes in Algerian university

22 March 2024 3,670 0 View

How can idownload MAUD software in my laptop?

how can i download MAUD software in my pc?

03 October 2023 8,919 3 View

Is there any Online database of phenolic compounds? (Except phenol-explorer) ?

I am searching for an online database of phenolic compounds extracted from plants which contains their UV spectra (or at least their λmax). In the database phenol-explorer, there is almost...

24 April 2023 3,774 3 View

Why the hydrophobic interactions are not linked to the interert residue in the Yasara software ?

Hello everyone, I have a question about the YASARA software. I want to discover the hydrophobic interactions between some protein residues. These interactions are not linked to the interest...

14 February 2023 4,936 1 View

Is there any relationship between Virilio's dromology approach and Hartmut's acceleration paradigm ?

I think loudly about the two concepts of time I.e : "dromology and acceleration".. through an interdisciplinary framework between philosophy and politics..

07 October 2022 6,194 0 View

Cooja - How to assign different services to a mote/node?

I need to create a network of 14 motes.each mote is either a service provider or service requester. A node provide a service or services.I want each mote to provide 3 services withS1 = 0.90, S2 =...

14 August 2022 7,486 1 View

Why autodock vina does not generate the dlg files for acids such as coumaric, caffeic, gallic ...etc?

Why autodock vina does not generate the dlg files foor acids such as coumaric, caffeic, gallic ...etc?

02 August 2022 5,239 2 View

PCR products from Yeast DNA (ITS) are not intense?

Dear Community, I extracted DNA from yeasts and performed a PCR with ITS primers. The PCR bands are not intense and do not allow sequencing. I've tried to extract DNA with a lithium protocol, but...

11 June 2022 503 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View