11 November 2017 8 4K Report

Hi,

Does anybody have any experience with histologically processing arterial plaque tissue which may contain calcified regions?

  • I understand decalcifying agents such as EDTA can be used however what techniques can be used if you want to keep the calcified region (without dislodging during microtome slicing) in your histological slices for staining?
  • Additionally, if anybody had a working protocol for decalcifying atherosclerotic tissue, it would be a great help?
  • All advice is welcome!

    Robbie

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