Histamine stbility during vacuum concentration?

Anasua Pal @Anasua-Pal

21 April 2022 0 6K Report

Hello,

Does anyone know if histamine is degraded during vacuum concentration? Do I need to add a protease inhibitor?

I want to perform a Histamine ELISA after vacuum concentration.

Any suggestions or information would be really helpfu.

Badges
Science topic

More Anasua Pal's questions See All

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Want to authenticate aquatic plant species Duckweed? Two University botanist are unable to confirm, any confirmed place where it can be done in India?

06 June 2024 5,845 0 View

How do we quantify the flow behavior in a drying drop?

Is it possible to quantify the flow without adding any polymeric beads? I'm interested to know whether there's dominant capillary or Marangoni flow. Aby suggestions would really be helpful.

05 May 2024 2,233 5 View

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week. How to fix?

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week..Dose anyone know how to fix it ??

19 April 2024 1,111 2 View

Alphascreen versus TR-FRET?

I want to quantify low cAMP levels generated of a constitutively active GPCR also by different mutants of this receptor. I have tried the cAMP ELISA kit from Cayman chemicals with very...

14 April 2024 7,319 0 View

Has AI taking a big leap in Science and Engineering?

AI is coming fast, and we have to adapt.

29 March 2024 2,315 1 View

Bone material: What this minimum of maximum strain of Human cortical Bone before fracture?

What this minimum of maximum strain of Human cortical Bone before fracture. I saw lot of papers showing different values.

27 March 2024 4,255 1 View

Does the presence of degenerate nucleotides in consensus sequences affect the results of population genetic analysis?

I want to run STRUCTURE on a nuclear gene of an insect species. The sequences that I have received are showing the presence of degenerate nucleotides in the consensus sequences. Do I need to edit...

29 February 2024 648 1 View

Why binding energy in XPS shifted to lower side (decrease in the value) in composite with respect to base material?

In composite material, two elements of the base material show a positive shift in the binding energy, while the third element shows a negative shift in the binding energy. What are the reasons for...

11 February 2024 367 2 View

How can i change bond type in pdb file?

Hi i am doing work on a protein which has Cu ion in it but the bond it is making with protein are not matching the reference protein making bond with Cu ion so is there any possible to change the...

23 January 2024 5,999 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How do you determine the Ki of an inhibitor for an enzyme which also undergoes substrate inhibition?

There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.

08 July 2024 4,988 1 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View

Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

05 June 2024 6,447 1 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Why are protease inhibitors necessary in denaturing conditions?

I know that conventional wisdom dictates that protease inhibitors should be added to lysis buffers when extracting protein to prevent sample degradation. That said, why are such inhibitors...

14 May 2024 2,145 2 View

Is trying to position an activator compound in the pocket where the inhibitor binds a targeted approach?

If a 3D protein is obtained with the inhibitor complex, its wild-type size may change completely. In this case, it seems correct to investigate the binding mode with the inhibitor molecule. If the...

26 April 2024 3,509 5 View

How to modelling MD of Covalent Inhibitor?

I'm designing a covalent inhibitor on an in silico study. Should I use the "pre-reaction" non-covalent ligand-protein complex or covalently bonded one on the Molecular Dynamic simulation input? I...

20 April 2024 5,662 2 View