Additional bands on Western blot after protein overexpression? What are the additional products?

Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...

26 November 2020 5,080 4 View

Has anyone pooled libraries from ChIP and RNA experiments in one lane for sequencing?

I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!

21 July 2020 1,846 4 View

Is any requirement to the compressed air clearness to use with the laser optics ?

I understand that the compressed air for the laser optics purge (first step of cleaning) such as mirrors or lenses should be "clean", but does anybody can explain me what really does it mean...

01 April 2019 8,021 5 View

Can anybody recommend me a company to buy graphite target useful for PLD technique?

I would like to buy graphite target for further DLC films creation by PLD technique, does anybody have some good experience with company offering it in terms of reliability and good...

14 March 2019 8,314 3 View

Source of DiFi cell line

Do you know where can I buy DiFi cell line? There are not available in ATCC, ECACC or DSMZ.

20 July 2017 3,772 1 View

Can I measure expression of MIR genes on Illumina BeadChip if I prepare cRNA using Ambion Total Prep Kit (based on oligodT primers)?

Because list of probes on the BeadChip contains some of MIR genes (i.e. MIR1-2, MIR1200, MIR1202, MIR1203 etc.) however, as I remember correctly - the mature microRNAs don't contain the polyA...

19 June 2016 2,867 3 View

Has anyone investigated the influence of the head coil type on resting state data?

What do you think about using 8-channel head array coil?

04 January 2014 8,779 2 View

Is there any GC-ECD in NUIG that can be adapt to measure N2O in a gas phase?

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26 November 2013 2,205 1 View

Role of Clostridium genus in gut brain axis. Investigation of neural connectivity loss in Autism Spectrum Disorder (ASD), nosology, treatment and ther

Dear fellows, I am currently working around specified subject. As part of my investigation I recruit volunteers, able to provide faecal tests results, suitable for ASD patients and control....

01 January 1970 6,630 3 View

Drama on the stage vs. new philology

I'm analysing the relation between the work with literary drama on the stage in the theatre and the theory of new philology (medieval texts). Do you have an example for it in the contemporary...

01 January 1970 8,329 2 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Which one carries more value? A research paper in a Scopus Indexed conference proceedings or the same paper in a Journal without Scopus indexing?

I am looking for the suggestion among which one to pick from the above two categories for a research work.

27 February 2021 10,025 1 View

Which indexation is better Scopus or Web of Science?

Both are very repetitive indexing agencies.

26 February 2021 9,455 4 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View