Hi, Why different bacteria genus or species use the different replicon in order to replication?

09 March 2015 1 5K Report

It mean that when i want to construct the shuttle vector for E.coli/Lactobacillus fermentum

http://www.google.com.eg/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&ved=0CCYQFjABahUKEwiCmO-06YPGAhUGBSwKHWBZABc&url=http%3A%2F%2Fwww.annmicro.unimi.it%2Ffull%2F54%2Fwild_54_471-480.pdf&ei=in53VYKrGoaKsAHgsoG4AQ&usg=AFQjCNE_7Rj-AsTbGPRZHqW2537t1bsrOQ&bvm=bv.95039771,d.bGg

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What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

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What is the exact mechanism of the antimicrobial effect of local anesthetics?

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How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

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24 February 2021 9,839 3 View

Errors in DNA sequences?

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Advice on growing lactobacillus species in the lab?

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24 February 2021 3,157 6 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

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How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

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Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View