What is the easy and low-risk synthesis of carboxymethyl cellulose from bacterial cellulose?
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
Hello everyone, I have a dataset of videos for action classification, where each video contains multiple actions. I need to annotate these videos with the name of each class and the start and end...
17 May 2024 5,293 2 View
I need to do western blot against H3K9me3 and I tested it once from my Protein extracted by Np-40 lysis buffer, I had a very nice band but the problem is the band was not at the right size (15...
11 February 2024 5,692 0 View
I would like to find a reliable reference that includes electronic properties such as CB & VB DoS, electron & hole mobilities, thermal velocities of semiconductors like ZnTe, CdTe. I am...
10 February 2024 7,803 4 View
Hi all I am studying a cationic cluster containing one molecule of methanol and one molecule of halobenzene, I am investigating a possible intra-cluster reaction leading to the formation of...
29 October 2023 6,171 6 View
Hello all, Regarding self-healing hydrogels, can only the covalent Schiff-base bond break and form quickly, or do other bonds give this self-healing property to the hydrogel?
28 October 2023 1,050 3 View
Hello all, I need a formula for solving this problem. I want to calculate SOBP in Proton therapy.
26 August 2023 1,912 3 View
does anyone knows if the MDL output format in Gaussian is the same as a molden output?
09 August 2023 3,936 1 View
Hi is there any way to convert Gaussian output file (fchk) to molpro output?
11 July 2023 1,543 7 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...
16 July 2024 4,698 4 View
Hi, I have grown BL21 cells with the pET28a that carries my insert sequence. It is growing on the selection plate but I can't observe any insert sequence bands when I do colony PCR. Is there any...
15 July 2024 5,135 5 View