Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

How do I can remove the cladding a hard-clad fiber with chemical method?

How do I can remove the cladding a hard-clad fiber with chemical method?

15 December 2019 8,888 3 View

Hi, i want to work in targeted cpp-mediated delivery of pDNA. but i do not know if DNA can stably reach to nucleus?

i want to design a cell penetration peptide for delivery of pDNA. that this platform targeted with a tumor targeting peptid

24 April 2019 6,763 2 View

What is the best method for fresh vegetable disinfection?

what is the best method for fresh vegetable washing, in order to remove pathogenic microorganisms and pesticides.

30 December 2018 8,370 3 View

How do analyse dynamic path in survival?

I want to write a program of dynamic path analysis in R software for estimating direct, indirect, and total effects of treatment on time of death due to liver cirrhosis . data-set is as " csl" in...

13 June 2016 1,488 1 View

What is the best method for chlorophyll extraction from olive oil?

liquid-liquid extraction of chlorophyll

12 February 2016 2,745 2 View

What is the best method to study gene-gene interaction?

I am looking for the most reliable method to study gene-gene interaction.

30 January 2016 5,522 8 View

What are steps of running CV test to check bioactivity of an anode or a cathode in MFC and MEC systems?

13 September 2015 747 3 View

Does anyone know how to fix the matter with floating particles in COD test?

Hi All, I will appreciate if anyone could share his/her experience or any info about this matter with me. I am doing COD test on some industrial waste samples. to make it economic, i make reagents...

27 March 2015 5,985 8 View

Can anyone recommend a cheap and handy technique to measure gas production and pressure in serum bottles?

Hi, I would appreciate if any one can help me to find a cheap and reliable technique to measure gas pressure and amount in a serum bottle?

14 March 2015 487 4 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View