Hi, pls can any one help me on how i can prepare master mix for a PCR reactionto for identification Acinetobacter species

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Muhammed Duman

You should read your PCR kit instruction or ready to use master mix instruction and must add Acinetobacter specific primer pairs into this mix. After, you must placed these tubes in thermal cycler and you should try some PCR condition that should be similar conditions in Acinetobacter researches.

Ahmad Bagudo

Hi Muhammad thanks a lot