Hi, is there a colorimetric method to determine amidase activity in soils?

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I meant other colorimetric method to determination amidase activity in soils without UO2(C2HgOg)2'2H2O reagent?

because i can not to get this chemical reagent in my country at least or minimum i will need special federal permissions... to use or to be tested.

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Eloy Conde

this is the reagent i can not get:

UO2(C2HgOg)2'2H2O

Bruce Neagle

Uranyl Acetate is used to determine the rotation of a corn starch/corn sugar solutions. I doubt, very much, your concentration is high enough to determine the amidase activity. You can try reviewing ICUMSA (International Commission of Uniform Methods of Sugar Analysis) methods.

You might try incubating a known concentration of starch in the soil and perform a back titration with Standard Iodine Solution (use KSCN to sharpen the endpoint).