Yes you can use. But mostly it depends upon the type of formulation. You can also opt for ultrasonicators but you have to be more careful about time which should be less than 4 minutes because more duration will burst the prepared nano-particles.
You can even go for 80% amplitude for preparation of nanoparticles depending on what type of nanoparticles your are working on .... in case of inorganic nanoparticles such as mesoporous, it has no effect on size and hence size reduction is not obtained..
In case of polymeric nanoparticles such as PLGA, PLA or PCL, the amplitude may affect the shape and size of nanoparticles but can also lead to decrease in entrapment efficiency if time is extended beyond 3 min in one cycle as at amplitude more than 60%, lot of heat is produced in solution which leads to increase in temperature of solution. If Tg for polymer is below 50 degree such as in case of some lipid for SLN and NLC then it will cause lysis of nanoparticles.
So, I will suggest before going for particular amplitude and time you should check the Tg of p0lymer and stability of drug.
In case of nanoparticles loaded with pDNA, siRNA protein and herbal extract such as flavanoid dont exceed 40% 3 min/3 cycle
ethyl cellulose if okk but in case of HPMC you need to be aware of the fact that HPMC starts becoming hydrophobic with increase in temperature and ultimately starts forming polymer aggregates above 50-55 degree. It can also form gel in combination with ethyl cellulose. So the best you can do is keep your beaker or eppendorf in ice cold water and then go for probe sonification. And make sure the temperature does not reach more than 53 degree... I guess this will work.
Thank you. I guess different probe sonicators have different Powers. Mine one has 500 watts, 20 khz and I am using it at 70 %.
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