HI! I I would like to know if there is a technical way to stain fresh adipose tissue without fixation with formaldehide?

Sharon Leigh Kracoff-Sella @Sharon-Kracoff-Sella

07 December 2022 0 2K Report

I would like to apply to the Adipose Tissue a live-dead stain for example

Badges
Science topic

More Sharon Leigh Kracoff-Sella's questions See All

How to correct my name?

How can I correct my name on my profile?

30 June 2024 10,166 1 View

Is it possible to use a antibody specific for IF in IHC? and if yes, which will be the protocol??

I have an antibody specific just for ICC and WB but I should use the same antibody to perform an IHC staining. Is it possible to use this ab also in IHC?

15 May 2024 6,392 3 View

Any existing technology for the conversion of butene/propylene to dodecene?

Exploring possible technologies offered by companies for the conversion of butene to dodecene by polymerization, with olefin conversion rate >90% and selectivity > 50%.

02 April 2024 6,546 0 View

Is polyglycolic acid being explored for its use as support material in 3D printing?

PGA seems to be promising with its biodegradability, is that driving its research for use as support material? Are PGA's completely soluble in water?

03 March 2024 6,917 1 View

Panel data Regression for unbalanced Data?

Hi Guys, I have been working with panel data for my research. One of the problem I faced is that. My dataset is monthly based from 2010 to 2023. I was able to do a yearly analysis but for monthly...

29 November 2023 5,140 0 View

Is Surangin (a coumarin) available in market as a hptlc marker? In India?

Surangin is a coumarin. Wanted to know if it is available in Indian market as a standard/marker?

25 October 2023 8,499 0 View

What is the difference between R and SPSS in estimating random effects?

Hello, I need to estimate a generalized linear mixed model (GLMM). When using R, I need to choose a specific fixed effect (one of the independent variables in the model) to have a random slope...

12 October 2023 6,438 3 View

How to measure goodness of fit for a mixed-effect logistic regression model?

Hello, I estimated a mixed-effect logistic regression model (GLMM) and I need to evaluate it. Specifically, I tried a few combinations of the independent variables in the model and I need to...

12 October 2023 9,391 3 View

How to decide whether to use non-parametric stats?

I have an anonymous survey with a sample of 325 people, asking emotive questions (e.g. emotions and perceived threat with regard to refugees). There was high skewness in some variables. But that...

10 October 2023 455 17 View

Where is VSM in working condition in Tamilnadu?

I want to analyse my samples in Vibrating Sample Magnetometer. M vs T and M vs H. I couldn't find VSM working anywhere in Tamilnadu right now. If anyone knows about it, please reply. If not in...

07 October 2023 898 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View