I would like to apply to the Adipose Tissue a live-dead stain for example
Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...
28 February 2021 1,369 5 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi all, I recently posted a question about how to conduct a convergent validity analysis for a self-developed self-esteem scale. I got some very useful responses! However, something I am stuck...
01 December 2020 4,370 6 View
The light fraction (undecomposed organic matter) needs to be removed from prepared soils (
02 June 2020 921 5 View
Kindergarten learners with internet connectivity at home are also encouraged to access enrichment activities. How mdifficult the new normal is, if connection is not possible, worst if there is no...
10 May 2020 10,019 3 View
As above, we are trying to do the Long-term potentiation test using mice hippocampal slices. How many mice are needed or how many slices are needed for LTP? Can anyone help me with that?
15 February 2020 7,073 3 View
Reason I ask is because I can find people that may be interested in the subject I have submitted my self to a fund raiser for autism on the Facebook network. any people interested in donating to...
27 December 2018 5,917 1 View
Hey, I have been trying to optimize the structure of what I guess I would call a lignin derivative. The system contains 63 atoms and has no charge. I have tried using DFT and HF using a 6-31g(d)...
06 November 2018 2,781 3 View
Hi, I would welcome some advise regarding the importance of the value of the coefficient in Hayes PROCESS Macro SPSS. In output 2, of the model, the mediator effect looks significant i.e.: (B =...
07 July 2018 8,116 2 View
These 'secretion-inhibited' cancer cells would then be co-cultured together with myeloid cells so I am hoping the environment would not be toxic to both cell types.
23 April 2018 7,624 8 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View