Hi, how do you go for Raman analysis for graphene quantum dots solution prepared using citric acid? Any suggestion?

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How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

What is the minimum number of cells required for fluorescence in situ hybridization?

I have to perform a FISH experiment with limited number of cells and I am trying to estimate the minimum number of cells required to do FISH. Cells are from mammalian cell line. I failed in doing...

12 January 2021 7,887 1 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

Can someone suggest me a wear detection method for a pipe which goes under intense heat and pressure at intervals?

I am looking for a wear detection method for a pipe that goes under intense heat and pressure for short instances at intervals. this process has an average cooldown time of 8 seconds between each...

08 October 2020 3,776 2 View

Single-cell cloning from SkBr3?

Did anyone tried single-cell cloning from SkBr3 cell line. I am failing in growing cells post single cell seeding in wells. Any help is appreciated

27 September 2020 8,762 4 View

What to do when RT PCR becomes positive again after 15-20 days of 1st sample positivity?

We are getting some patients who were initially positive of RT PCR for Covid. But in retesting after 15-20 days or even 20 days after those patients remain still positive. Is it for dead RNA...

17 August 2020 9,552 9 View

How to calculate % sequence identity using Clustal W?

I am calculating % epitope conservation for different mammalian antibodies against zebrafish. I have performed the Clustal W for antigenic peptide used in raising the antibodies against the...

05 August 2020 6,399 5 View

What is the Source of Harmonic Power ?

Consider a sinusoidal supply at fundamental frequency as shown, with a series inductive reactance leading to a bus. Let a diode rectifier load be connected to the bus. The switch Sw for resistive...

24 June 2020 8,175 27 View

What is the best book on Game theory from the perspective of Computer Science and Engineering?

I do not know much about game theory. I am finding a book where different protocols and algorithms of computer science have been analyzed using the concept of game theory.

17 May 2020 4,774 7 View

How can a PhD in interdisciplinary fields can benefit a researcher in job market?

I am a PhD scholar of Humanities and my research is broadly connected to Anthropological studies and framework. I have also published with an Anthropological journal of global repute and looking...

10 May 2020 4,202 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View