PEG coated Fe3O4 nanoparticles in aqueous NH3?

Hello, I synthesized PEG coated Fe3O4 nanoparticles by coprecipitation methods and I would like to ask whether they can be dispersed in aqueous NH3 since they have a PEG coating and theoretically...

06 February 2021 5,217 4 View

Is there such a function? Is it unique?

21 January 2021 8,962 13 View

What are the current developments in terms research and publications in anthropology in general and social anthropology in particular?

I am interested in examples of anthropological methods that have been applied across different disciplines, for exmple in mathematics education.

20 January 2021 3,090 3 View

How did the tests or exams get to Latin America, specifically the written tests?

I am looking for some bibliographic references that explain how tests or exams (measurement and assessment in education) came to Latin America, specifically written tests. I would like to know if...

13 January 2021 8,607 0 View

Is this true for all values c > 0?

25 December 2020 3,621 45 View

Can children from 1 year old to 15 years be given MODANA and Pfizer Anti-COVID-19 vaccine?

The clinical disease of COVID-19 has been low in children generally and vaccine has been develop.Any evidence of its safety,efficacy and risk for allergy in children? any exemption for children...

23 December 2020 7,177 12 View

Can large samples be stored at -20 degrees prior to RNA extraction?

As part of a Covid project we are sampling urban rodents and performing RNA extractions and serology work. We have too many samples to store at -80 and have opted to perform extractions the same...

15 December 2020 5,252 2 View

Substrate functionalization and antibody deposition for biosensor work?

Hello, I am a bio student working in a lab on his free time and I just started working with an engineering group on sensors. My questions are: 1)Functionalization steps and what are the best...

21 November 2020 7,996 2 View

Why am I seeing RED fluorescence in control non transgenic plants?

Hi experts, I have cloned the DsRED gene into a plant vector under 35S promoter and used it for direct seed transformation of Chenopodium quinoa. When I checked the potential transformed...

24 October 2020 4,775 3 View

Do you think there are any prospects for hydrogen as an alternative to natural gas when operating a gas turbine?

At the moment it is very expensive, the price of 1 kg of hydrogen is from $ 2-5. Are there any prospects for green hydrogen? or will we extract hydrogen from gas? what promising technologies can...

19 October 2020 1,954 7 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View

Why fouled membrane has more negative zeta potential and lower IP?

I modified PES membranes with nanoparticles (nanosilver, graphene, halloysite). They were used in UF for WWTP effluent treatment. After filtration, all membranes exhibited more negative zeta...

26 December 2020 5,676 3 View

Is it possible to calculate the sample size of a clinical trial with the Cohen formula when there is no similar article?

We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...

21 December 2020 2,454 3 View

Has anyone tried cross-linking cells with formaldehyde before co-immunoprecipitation?

Hi, I am currently trying to co-immunoprecipitate a protein but the interaction between that protein and its binding partner is not stable. Sometimes we able to detect its binding partner but...

29 November 2020 9,261 3 View