Hi everyone, I want to ask how exactly the myocard works?

More Iveta Tóthová's questions See All

How to change filter cube slider in Inversed Fluoresence Microscope NIB-100F?

Can someone advice how to change slider with filter cubes in Inversed Fluoresence Microscope NIB-100F? This information is not provided in user manual and support is not responsive. With the best...

06 October 2021 9,263 1 View

How to do multiobjective nonlinear regression / least squares (in Python)?

I have been trying to find a way to fit two functions simultaneously using nonlinear least squares (I have to find the optimum 3 variables, common for both models, that fits best both of them). I...

19 July 2021 2,459 4 View

Do high-intensity workouts give more benefit rather than endurance?

According to the latest researches I tend to think that much more benefits in shorter time are reached by high-intensity trainings in the comparation with the traditional cardio activities. If I...

24 November 2015 5,566 5 View

Does anyone have experience with immunofluorescence of endoplasmatic reticulum?

Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...

06 June 2024 7,764 1 View

What type of inhibition does a Ca2+ chelator have?

As I remember according the classical enzymology there are four types of inhibition: Competitive, Non-competitive, Uncompetitive and allosteric Inhibition . There is an enzyme with Ca2+ as a...

21 March 2024 7,334 4 View

Has anyone worked on endoplasmic reticulum stress pathway in the cardiac fiborplast??

i would love to know for some direction in basic experimental research

07 January 2024 2,177 0 View

How can I determine the appropriate concentration of Ca2+ chelator (BAPTA-AM) to pre-treat plants (rice) for studying intracellular Ca2+ signaling?

I am interested in mapping the concentration of intracellular Ca2+ in rice plant cells. As a control treatment, I plan to apply the Ca2+ chelator BAPTA-AM. If anyone here has experience using Ca2+...

05 December 2023 7,381 0 View

How can I increase the intracellular Ca2+ level in the E.coli???

I want to see how the intracellular calcium level affect the comformation of protein in E.coli. Can I just add the Ca into the culture media? or if there are some useful ionophore and if you know...

28 September 2023 3,426 0 View

Will the cytosolic Ca2+ of myoblast elevated during the initiation of differentiation?

Someone said that transient Ca2+ depletion from the endoplasmic reticulum is critical for skeletal myoblast differentiation. I found that one gene deficiency in myoblast leads to precocious...

28 August 2023 2,916 0 View

Is it possible to isolate the endoplasmic reticulum (ER) and Golgi body using subcellular fractionation?

I am trying to find a decent protocol, but the only ones I see are for generating nuclear, mitochondrial, and cytoplasmic fractions.

13 August 2023 3,187 1 View

Intracellular calcium mobilization assay experiences in Varioskan LUX?

I am planning to perform intracellular Ca2+ mobilization assays on 96-well plates using EndoC-βH1 cells. Specifically, I am interested in measuring β-cell responses to glucose and possibly other...

16 July 2023 3,886 0 View

How important is flow for ca2+ analysis in cells in response to a drug?

Hi, I am studying the Ca2+ response on hCMEC/D3 cells and have been adding my drug of interest with a pipette (at the speed of about 50-100uL/sec). However this addition is not in control and I...

10 July 2023 7,415 0 View

Hi,guys! I want to ask if any of you would know about mitochondrial Ca2+ imaging in vivo?

mitochondrial Ca2+ imaging with two-photon microscopy for drosophila in vivo

30 May 2023 5,243 0 View