I am trying to measure cellulose, hemicellulose and lignin in residual lignocellulosic material. I need an easy and effective method. Thanks!
Probably it's difficult to achieve everything "simple, easy, cheap...", Nicolas...!? - we're using ANKOM2000 fiber analyser (ANKOM Technology, USA) to measure the ADF, NDF and ADL content according to the Weender analysis.
You can look for NREL protocols for Lignin and sugars analysis (attached). Its a simple 72% acid hydrolysis and need HPLC for residual sugars and you can calculate cellulose n hemicellulose content! Good Luck.
None other method is as simple as suggested by Dr. Rashmi. Good luck!
Method suggested by Kataria is ok if you dont have the easiest, simple analyser suggested by Dr Joachim Venus. Other conventional methods exists which are equally good, but time consuming and energy sapping. Good luck.
Agree with the above comments for a "one-time" or infrequent analysis. If this becomes a regular / routine test for you, vis-NIR analysis is rapid and can provide accurate determination of cellulose, hemicellulose, lignin, extractives, ash, and moisture content once a material-specific initial calibration is performed. Samples tested in minutes, not hours. Dr. Keith Luo at Alberta Innovates - Technology Futures in Edmonton, Canada is the best person to discuss.
https://www.linkedin.com/in/qi-keith-luo-ab963272
You may follow TAPPI standards for all you biomass characterization methods
Hi nicolas,
You can use folloving article to analyse the cellulose, hemicellulose, lignin, and extractives
"Fast Pyrolysis of Biomass in Free-Fall Reactor for Hydrogen-Rich Gas"
Good luck.
For storage of E. coli for four to five days only? Storage at +4°C is just perfect.
Streak the E. coli on agar plates wrapped with paraffin and store upside down. This will minimize contamination as well as aid keep both the culture and agar properly hydrated.
Best of luck!
You can use the ASTM or TAPPI standars for your chemical analysis
you can use Wise Chloride Method for holocellulose, Kurschner - Hoffer's nitric acid method for cellulose and TAPPI for lignin
Yes NIR ( near infrared spectroscopy method) .Lot of work has been done on this line .I also did work for Leuceana leucocephala (subabul? and Sarkanda under NIMILIT project sponsored by CSIR
You can contact DrSK.Shukla IWST Bangalore IndiI retired scientist
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