If you have any exprience or references useful for beginners to use this method I am highly appreciated if you could share with me.
Hi John,
Thank you for your comments.
1. it is the first time that I i want to use LC/MS
2. I am going to analyse lipid profiles and bile acids
3. I have no expreince to work with any types of LC
4. I am going to operate the instrument
5.yes I have a mentor but before I meet him I want to become familiar to know more fundamental details
6.trouble-shooting is my second priority I shall be familiar with the principal of method
7.there is no available training at the moment if I want to attend in the company. the instrument is Agilent 6420
I am highly appreciated if you share your knowledge with me
Best Regards
Reza
Can you tell, which LC-MS instrument are you going to use? Is it Agilent or Waters or Shimadzu? Are both LC and MS instruments from same manufacturer or LC from one manufacturer and MS from another? For e.g. Agilent LC coupled to Waters MS or Agilent LC coupled to Shimadzu MS or Waters LC with Agilent MS etc.? For lipids' analyses, with respect to LC, choice of mobile phase (solvents) is important. Do you have any prior knowledge of HPLC? It might help to design some experiments of the LC part. With regard to MS, you need to read. For Mass Spectrometry, you may try to get and read the following two books: 1. "Mass Spectrometry. A Textbook" 2nd edition by Jurgen H. Gross. 2. "Mass Spectrometry. Principles and Applications" 3rd edition by Edmond de Hoffmann and Vincent Stroobant. From these books or from a reliable internet source, you need to understand the basic functioning principle of Quadrupole. Subsequently, you may be able to understand the functioning concepts of Triple Quadrupole.
http://as.wiley.com/WileyCDA/WileyTitle/productCd-047003310X.html
http://www.dmf.unicatt.it/~gavioli/corsi/MSFM/ref/Vuoto+Camere/Mass_Spectrometry__A_Textbook.pdf
Hi Dear Sabareesh
thank you for the book. As I mentioned above I will be using Agilent 6420
regards
Reza
I have found the Hoffmann & Stroobant book very useful so please do look at that. It may be worth looking for Societies to join which may mean you get reductions in the costs of 'beginner' courses on Mass Spec (usually run by the society you have joined). Other than that the resources and advice of John Wishnok above are incredibly valid and useful.
go on youtube to learn about it. Lots of lectures were presented. Company put lots of video so you understand of what is going on inside the instrument. I wish I had this 20 years ago.
Agilent has several instructional videos for new users of the instruments that comes with the software. Some are also available on the Agilent website.
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