How to calculate the crossliking density of the rubber?

How to calculate crosslinking density of the rubber and Molecular weight between crosslinks in NR, SBR, etc..

27 June 2017 5,642 5 View

What is the role of polarity of the solvent in the emission behavior?

01 November 2016 712 10 View

How does Ceiling voltage of the Excitation system affect the gain of the AVR?

We understand that the gain of the PID or lead lag block of the AVR is factor of Ceiling voltage of the excitation system.Please explain that how ceiling factor affects the gain and improves...

12 October 2016 2,165 2 View

What causes colour changes of pectin during acid hydrolysis?

I did acid hydrolysis to breakdown pectin into monosaccharides for sugar analysis. The colour of pectin eventually changes into the colour of the fruit itself during the hydrolysis. Wondering what...

14 September 2016 8,540 1 View

What are the best system specifications required for running GROMACS lipid bilayer simulations?

21 August 2016 6,467 3 View

Am useing 1:20 ratio (w/v) for hydrolysis of corncob powder and getting 10gL xylose.,how to calculate percentage acid hydrolysis in biomass?

kindly help me to calculate percentage acid hydrolysis of corncobs with sulphuric acid (1%), am getting 10gL Xylose during process

25 July 2016 4,007 3 View

Can anyone assist me to interpret the correlation between DPPH assay and FRAP assay?

I have extracted plant samples using four different solvents. I found that the DPPH scavenging activity of all tested samples are averagely similar but the FRAP reducing result of a particular...

23 November 2015 5,967 2 View

What's the difference between aldoketo reductase and aldo-keto reductase?

I am looking for phylogenetic analysis is aldoketo reductase family protein, while searching the sequence related to the same in NCBI. Aldo-keto reductase and aldoketo reductase giving different...

22 February 2015 2,551 3 View

How can I calculate mass gain in TGA tests?

We were conducting TGA tests for a Mg Alloy system. The Y Axis that determined TG% starts its value from 100 and the curve that i got increased upto a value nearing 165-170 in Y Axis and then...

27 January 2015 7,861 2 View

Why is searching articles using first AND last name in pubmed not easy?

24 December 2014 3,606 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

How to define fuel mixture as input in ansys fluent species transport model ?

I want to define a CO + H2 mixture fuel for a combustor. How can I define this in ansys fluent mixture template ? Normal database does not include this mixture . eg. if I want to define a mixture...

01 February 2021 6,860 2 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View